Hinf I endonuclease digestion + Giemsa staining (a) on bovine chromosomes and subsequent C-banding (b). Note the differential staining of heterochromatic blocks and subsets.
The same procedure using Taq I endonuclease
Reference: Hidas, A.: Heterochromatin heterogeneity revealed by restriction endonuclease digestion and subsequent C-banding on bovine metaphase chromosomes. Hereditas 1995. 122. 285-288.
In situ nick translation a sensitive method for detection of not onlyDNA extraction, but also cleveage.
Dra I digestion and in situ nick translation on bovine chromosomes. DNA of all the heterochromatic segments are uncut (there are no fluorescent signals)
Hinf I digestion and in situ nick translation on a partial bovine metaphase. Heterochromatic segments are mostly cut with the exceptions of some subsets (marked)